Original Article
JBCGenetics. 2021; 4(2): 76-84

Effect of vitamin D3 supplementation upon the metabolic and DNA methylation profile of cystic fibrosis patients

Authors: Maria Paula de Paiva, Dayanna Joyce Marques Queiroz, Celso Costa da Silva Junior, Constantino Giovanni Braga Cartaxo, Marina de Castro Coelho, Rafaella Cristhine Luna Pordeus, Rafaela Lira Formiga Cavalcanti de Lima, Darlene Camati Persuhn, Alexandre Sergio Silva, Naila Francis Paulo de Oliveira, Maria da Conceicao Rodrigues Goncalves.

View PDF HTML Fulltext DOI: 10.24911/JBCGenetics/183-1613502878

Abstract

Background: Cystic fibrosis (CF) is a genetic disease that affects the transmembrane conductance regulator gene responsible for modulating chloride ion transportation in the cell membrane. Hypovitaminosis D is frequently observed among fibrocystic disease patients. Therefore, this study was aimed to evaluate the effect of vitamin D3 supplementation in patients with CF concerning their metabolic and deoxyribonucleic acid (DNA) methylation profiles. Methodology: A clinical trial involving 12 CF patients was carried out in Joao Pessoa. After assessment of hypovitaminosis D prevalence in the studied population, four patients with vitamin D3 insufficiency/deficiency were administered cholecalciferol megadose supplementation in addition to biochemical examinations and analysis of inflammatory and epigenetic indicators. The DNA methylation profile of the studied genes' promoter regions was determined through a qualitative methylation restriction enzyme technique. Data were analyzed using the Statistical Package for the Social Sciences 25.0 software for T-tests, Mann-Whitney, and Wilcoxon test calculations. Results: Hypovitaminosis D was observed in 58%, 33% of the studied individuals. Patients with hypovitaminosis D reported blood sugar, glutamic-pyruvic transaminase (ALT), and uric acid levels significantly higher (p = 0.02; p = 0.05; p = 0.02, respectively) compared to individuals with sufficient 25-hydroxyvitamin D (25(OH)D), as well as elevated inflammatory values. Supplementation did not influence epigenetic nor metabolic parameters significantly, although the mean 25(OH)D serum concentration value increased from 18.3 ng/dl to 34.1 ng/dl (p = 0.06). Conclusion: Cholecalciferol megadose elevated 25(OH)D serum levels, although it did not alter inflammatory, glycemic, or epigenetic parameters. This encourages future studies on the matter since significant differences were found in blood sugar, uric acid, and ALT serum levels for the vitamin D3 insufficiency/deficiency group despite this study’s small sample size.

Keywords:   Epigenetics, cystic fibrosis, supplementation, vitamin D, DNA methylation


© Copyright: Author(s)

Effect of vitamin D3 supplementation upon the metabolic and DNA methylation profile of cystic fibrosis patients


Authors
Maria Paula de Paiva
Department of Nutrition Sciences, Federal University of Paraiba, Brazil
PubMed articlesGoogle scholar articles

Dayanna Joyce Marques Queiroz
Department of Nutrition Sciences, Federal University of Paraiba, Brazil
PubMed articlesGoogle scholar articles

Celso Costa da Silva Junior
Department of Nutrition Sciences, Federal University of Paraiba, Brazil
PubMed articlesGoogle scholar articles

Constantino Giovanni Braga Cartaxo
Hospital Universitário Lauro Wanderley, Federal University of Paraíba, Brazil
PubMed articlesGoogle scholar articles

Marina de Castro Coelho
Federal University of Piaul, Brazil
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Rafaella Cristhine Luna Pordeus
Federal University of Piaul, Brazil
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Rafaela Lira Formiga Cavalcanti de Lima
Department of Nutrition Sciences, Federal University of Paraiba, Brazil
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Darlene Camati Persuhn
Department of Nutrition, Federal University of Paraiba, Brazil
PubMed articlesGoogle scholar articles

Alexandre Sergio Silva
Department of Nutrition Sciences, Federal University of Paraíba, Brazil
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Naila Francis Paulo de Oliveira
Department of Nutrition, Federal University of Paraíba, Brazil
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Maria da Conceicao Rodrigues Goncalves
Department of Nutrition Sciences, Federal University of Paraiba, Brazil
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Correspondence to:
. Maria Paula de Paiva, Graduate Program in Nutrition Sciences, Federal University of Paraiba, Brazil.; nutripauladepaiva@gmail.com

Publication history
Received 16 Feb 2021
Revised 25 May 2021
Accepted 26 May 2021
Published online 27 Jul 2021
Published in print 28 Aug 2021

How to cite this article

Pubmed Style

Paiva MPd, Queiroz DJM, Junior CCdS, Cartaxo CGB, Coelho MdC, Pordeus RCL, , Persuhn DC, Silva AS, Oliveira NFPd, Goncalves MdCR. Effect of vitamin D3 supplementation upon the metabolic and DNA methylation profile of cystic fibrosis patients. JBCGenetics. 2021; 4(2): 76-84. doi:10.24911/JBCGenetics/183-1613502878


Web Style

Paiva MPd, Queiroz DJM, Junior CCdS, Cartaxo CGB, Coelho MdC, Pordeus RCL, , Persuhn DC, Silva AS, Oliveira NFPd, Goncalves MdCR. Effect of vitamin D3 supplementation upon the metabolic and DNA methylation profile of cystic fibrosis patients. https://www.jbcgenetics.com//?mno=56552 [Access: December 08, 2021]. doi:10.24911/JBCGenetics/183-1613502878


AMA (American Medical Association) Style

Paiva MPd, Queiroz DJM, Junior CCdS, Cartaxo CGB, Coelho MdC, Pordeus RCL, , Persuhn DC, Silva AS, Oliveira NFPd, Goncalves MdCR. Effect of vitamin D3 supplementation upon the metabolic and DNA methylation profile of cystic fibrosis patients. JBCGenetics. 2021; 4(2): 76-84. doi:10.24911/JBCGenetics/183-1613502878


Vancouver/ICMJE Style

Paiva MPd, Queiroz DJM, Junior CCdS, Cartaxo CGB, Coelho MdC, Pordeus RCL, , Persuhn DC, Silva AS, Oliveira NFPd, Goncalves MdCR. Effect of vitamin D3 supplementation upon the metabolic and DNA methylation profile of cystic fibrosis patients. JBCGenetics. (2021), [cited December 08, 2021]; 4(2): 76-84. doi:10.24911/JBCGenetics/183-1613502878


Harvard Style

Paiva, M. P. d., Queiroz, . D. J. M., Junior, . C. C. d. S., Cartaxo, . C. G. B., Coelho, . M. d. C., Pordeus, . R. C. L., , Persuhn, . D. C., Silva, . A. S., Oliveira, . N. F. P. d. & Goncalves, . M. d. C. R. (2021) Effect of vitamin D3 supplementation upon the metabolic and DNA methylation profile of cystic fibrosis patients. JBCGenetics, 4 (2), 76-84. doi:10.24911/JBCGenetics/183-1613502878


Turabian Style

Paiva, Maria Paula de, Dayanna Joyce Marques Queiroz, Celso Costa da Silva Junior, Constantino Giovanni Braga Cartaxo, Marina de Castro Coelho, Rafaella Cristhine Luna Pordeus, Rafaela Lira Formiga Cavalcanti de Lima, Darlene Camati Persuhn, Alexandre Sergio Silva, Naila Francis Paulo de Oliveira, and Maria da Conceicao Rodrigues Goncalves. 2021. Effect of vitamin D3 supplementation upon the metabolic and DNA methylation profile of cystic fibrosis patients. Journal of Biochemical and Clinical Genetics, 4 (2), 76-84. doi:10.24911/JBCGenetics/183-1613502878


Chicago Style

Paiva, Maria Paula de, Dayanna Joyce Marques Queiroz, Celso Costa da Silva Junior, Constantino Giovanni Braga Cartaxo, Marina de Castro Coelho, Rafaella Cristhine Luna Pordeus, Rafaela Lira Formiga Cavalcanti de Lima, Darlene Camati Persuhn, Alexandre Sergio Silva, Naila Francis Paulo de Oliveira, and Maria da Conceicao Rodrigues Goncalves. "Effect of vitamin D3 supplementation upon the metabolic and DNA methylation profile of cystic fibrosis patients." Journal of Biochemical and Clinical Genetics 4 (2021), 76-84. doi:10.24911/JBCGenetics/183-1613502878


MLA (The Modern Language Association) Style

Paiva, Maria Paula de, Dayanna Joyce Marques Queiroz, Celso Costa da Silva Junior, Constantino Giovanni Braga Cartaxo, Marina de Castro Coelho, Rafaella Cristhine Luna Pordeus, Rafaela Lira Formiga Cavalcanti de Lima, Darlene Camati Persuhn, Alexandre Sergio Silva, Naila Francis Paulo de Oliveira, and Maria da Conceicao Rodrigues Goncalves. "Effect of vitamin D3 supplementation upon the metabolic and DNA methylation profile of cystic fibrosis patients." Journal of Biochemical and Clinical Genetics 4.2 (2021), 76-84. Print. doi:10.24911/JBCGenetics/183-1613502878


APA (American Psychological Association) Style

Paiva, M. P. d., Queiroz, . D. J. M., Junior, . C. C. d. S., Cartaxo, . C. G. B., Coelho, . M. d. C., Pordeus, . R. C. L., , Persuhn, . D. C., Silva, . A. S., Oliveira, . N. F. P. d. & Goncalves, . M. d. C. R. (2021) Effect of vitamin D3 supplementation upon the metabolic and DNA methylation profile of cystic fibrosis patients. Journal of Biochemical and Clinical Genetics, 4 (2), 76-84. doi:10.24911/JBCGenetics/183-1613502878


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