Original Article

Published: Dec 07, 2025 | DOI: 10.24911/JBCGenetics.11-2336

Novel Variant in CBP domain of GLI3 underlying Postaxial Polydactyly


Authors: Aneela Hazoor , Bibi Ayesha Zehri , Nadeem Hameed , Muhammad Sharif Hasni , Mehraj Gul orcid logo


Article Info

Authors

Aneela Hazoor

Institute of Biochemistry, University of Balochistan, Pakistan

Bibi Ayesha Zehri

Makran Medical College, Turbat, Kech, Balochistan, Pakistan

Nadeem Hameed

Shaheed Nawab Ghous Bakhsh Raisani Memorial Hospital Mastung, Balochistan, Pakistan

Muhammad Sharif Hasni

Institute of Biochemistry, University of Balochistan, Pakistan

Mehraj Gul

Biological Production Division, National Institute of Health, Islamabad, Pakistan

orcid logo ORCID

Publication History

Received: August 15, 2025

Accepted: October 14, 2025

Published: December 07, 2025


Abstract


Background: Polydactyly is common congenital limb anomaly characterized by having extra fingers or toes and can be either syndromic or non-syndromic. GLI3 plays an important role in limb development via the Sonic Hedgehog signaling system. 
Methods and results: In this study, we examined a Pakistan family with postaxial polydactyly in an autosomal dominant manner. Clinical assessment confirmed non-syndromic presentation without further systemic abnormalities. Whole exome sequencing and Sanger validation identified a novel heterozygous missense variant in GLI3 (Glioma-associated oncogene homolog 3) [NM_000168.6: c.3199C>T; p. (Pro1067Ser)].
Conclusion: This novel variant expands the GLI3 mutation spectrum and highlights the importance of comprehensive genetic screening for accurate diagnosis and counselling of families with isolated polydactyly.


Keywords: GLI3, Missense mutation, Polydactyly, Whole exome sequencing


Pubmed Style

Aneela Hazoor, Bibi Ayesha Zehri, Nadeem Hameed, Muhammad Sharif Hasni, Mehraj Gul. Novel Variant in CBP domain of GLI3 underlying Postaxial Polydactyly. JBC Genetics. 2025; 07 (December 2025): -. doi:10.24911/JBCGenetics.11-2336

Web Style

Aneela Hazoor, Bibi Ayesha Zehri, Nadeem Hameed, Muhammad Sharif Hasni, Mehraj Gul. Novel Variant in CBP domain of GLI3 underlying Postaxial Polydactyly. https://jbcgenetics.com/articles/2336 [Access: December 11, 2025]. doi:10.24911/JBCGenetics.11-2336

AMA (American Medical Association) Style

Aneela Hazoor, Bibi Ayesha Zehri, Nadeem Hameed, Muhammad Sharif Hasni, Mehraj Gul. Novel Variant in CBP domain of GLI3 underlying Postaxial Polydactyly. JBC Genetics. 2025; 07 (December 2025): -. doi:10.24911/JBCGenetics.11-2336

Vancouver/ICMJE Style

Aneela Hazoor, Bibi Ayesha Zehri, Nadeem Hameed, Muhammad Sharif Hasni, Mehraj Gul. Novel Variant in CBP domain of GLI3 underlying Postaxial Polydactyly. JBC Genetics. (2025), [cited December 11, 2025]; 07 (December 2025): -. doi:10.24911/JBCGenetics.11-2336

Harvard Style

Aneela Hazoor, Bibi Ayesha Zehri, Nadeem Hameed, Muhammad Sharif Hasni, Mehraj Gul (2025) Novel Variant in CBP domain of GLI3 underlying Postaxial Polydactyly. JBC Genetics, 07 (December 2025): -. doi:10.24911/JBCGenetics.11-2336

Chicago Style

Aneela Hazoor, Bibi Ayesha Zehri, Nadeem Hameed, Muhammad Sharif Hasni, Mehraj Gul. "Novel Variant in CBP domain of GLI3 underlying Postaxial Polydactyly." 07 (2025), -. doi:10.24911/JBCGenetics.11-2336

MLA (The Modern Language Association) Style

Aneela Hazoor, Bibi Ayesha Zehri, Nadeem Hameed, Muhammad Sharif Hasni, Mehraj Gul. "Novel Variant in CBP domain of GLI3 underlying Postaxial Polydactyly." 07.December 2025 (2025), -. Print. doi:10.24911/JBCGenetics.11-2336

APA (American Psychological Association) Style

Aneela Hazoor, Bibi Ayesha Zehri, Nadeem Hameed, Muhammad Sharif Hasni, Mehraj Gul (2025) Novel Variant in CBP domain of GLI3 underlying Postaxial Polydactyly. , 07 (December 2025), -. doi:10.24911/JBCGenetics.11-2336