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Original Article

Online Publishing Date:
01 / 01 / 2018

 


Use of HPLC-UV method for the analysis of maple syrup urine disease in plasma sample first time in Saudi Arabia

Abdul Rafiq Khan, Ali Al-Othaim, Ahmed Al-Fares, Najla Al Hussain.


Abstract
Background: Measurement of branched-chain amino acids (BCAAs) [valine (Val), alloisoleucine (allo-Ile), isoleucine (Ile), and leucine (Leu)] in plasma and dry blood spot samples is important for the diagnosis and monitoring of maple syrup urine disease (MSUD), which is a metabolic disorder that affects metabolism of BCAAs. We used a temporally short program on an High performance liquid chromatography (HPLC) (Biochrom 30) for the first time in Saudi Arabia to analyze plasma samples for the diagnosis of MSUD.
Methodology: 500 μl of plasma were mixed with 500 μl of 10% sulphosalicylic acid, followed by shaking for 5-10 seconds on a vortexer. The mixture was incubated at 4 C for 10 minutes followed by centrifugation at 10,000 rpm for 5 minutes. Supernatants were transferred into HPLC vials for injection.
Results: For all amino acids, the limit of detection and limit of quantification were 5.0 and 10.0 μmol/l, respectively. The method was linear in the range of 10-2,500 μmol/l. The method was specific and selective for the detection of BCAAs, including alloisoleucine. The correlation coefficient (CV) of method comparison was greater than 0.980 and percent recovery of all amino acids in plasma was 80%-100%, which was evaluated at two amino acid concentrations. Precision was assessed by repeat analysis of spiked plasma samples and percent CV was found to be <8% for all amino acids tested at two concentrations.
Conclusion: The short program of amino acid analysis was helpful for the early diagnosis of MSUD.

Key words: MSUD HPLC analysis


 
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Pubmed Style

Khan AR, Al-Othaim A, Al-Fares A, Hussain NA. Use of HPLC-UV method for the analysis of maple syrup urine disease in plasma sample first time in Saudi Arabia. JBCGenetics. 2018; 1(1): 26-30. doi:10.24911/JBCGenetics/183-1530358447

Web Style

Khan AR, Al-Othaim A, Al-Fares A, Hussain NA. Use of HPLC-UV method for the analysis of maple syrup urine disease in plasma sample first time in Saudi Arabia. https://www.jbcgenetics.com/?mno=302642493 [Access: March 13, 2024]. doi:10.24911/JBCGenetics/183-1530358447

AMA (American Medical Association) Style

Khan AR, Al-Othaim A, Al-Fares A, Hussain NA. Use of HPLC-UV method for the analysis of maple syrup urine disease in plasma sample first time in Saudi Arabia. JBCGenetics. 2018; 1(1): 26-30. doi:10.24911/JBCGenetics/183-1530358447


Vancouver/ICMJE Style

Khan AR, Al-Othaim A, Al-Fares A, Hussain NA. Use of HPLC-UV method for the analysis of maple syrup urine disease in plasma sample first time in Saudi Arabia. JBCGenetics. (2018), [cited March 13, 2024]; 1(1): 26-30. doi:10.24911/JBCGenetics/183-1530358447


Harvard Style

Khan, A. R., Al-Othaim, . A., Al-Fares, . A. & Hussain, . N. A. (2018) Use of HPLC-UV method for the analysis of maple syrup urine disease in plasma sample first time in Saudi Arabia. JBCGenetics, 1 (1), 26-30. doi:10.24911/JBCGenetics/183-1530358447


Turabian Style

Khan, Abdul Rafiq, Ali Al-Othaim, Ahmed Al-Fares, and Najla Al Hussain. 2018. Use of HPLC-UV method for the analysis of maple syrup urine disease in plasma sample first time in Saudi Arabia. Journal of Biochemical and Clinical Genetics, 1 (1), 26-30. doi:10.24911/JBCGenetics/183-1530358447


Chicago Style

Khan, Abdul Rafiq, Ali Al-Othaim, Ahmed Al-Fares, and Najla Al Hussain. "Use of HPLC-UV method for the analysis of maple syrup urine disease in plasma sample first time in Saudi Arabia." Journal of Biochemical and Clinical Genetics 1 (2018), 26-30. doi:10.24911/JBCGenetics/183-1530358447


MLA (The Modern Language Association) Style

Khan, Abdul Rafiq, Ali Al-Othaim, Ahmed Al-Fares, and Najla Al Hussain. "Use of HPLC-UV method for the analysis of maple syrup urine disease in plasma sample first time in Saudi Arabia." Journal of Biochemical and Clinical Genetics 1.1 (2018), 26-30. Print. doi:10.24911/JBCGenetics/183-1530358447


APA (American Psychological Association) Style

Khan, A. R., Al-Othaim, . A., Al-Fares, . A. & Hussain, . N. A. (2018) Use of HPLC-UV method for the analysis of maple syrup urine disease in plasma sample first time in Saudi Arabia. Journal of Biochemical and Clinical Genetics, 1 (1), 26-30. doi:10.24911/JBCGenetics/183-1530358447




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